RNAi Timeline
Timeline
Organism
Reference
1995
Guo S, and Kemphues KJ.
First noticed that sense RNA was as effective as antisense RNA for suppressing gene expression in worm
1998
Fire A, et al.
First described RNAi phenomenon in C. elegans by injecting dsRNA into C. elegans which led to an efficient sequence-specific silencing and coined the term "RNA Interference".
1999
Hamilton AJ and Baulcombe DC.
Reported posttranscriptional gene silencing (PTGS) in plants
Plants
2000
Zamore PD, et al.
Reported processing of long dsRNA by Rnase III (Dicer) into shorter fragments of 21-23-nt intervals in Drosophila extracts
2001
Bernstein E, et al.
Cloned Dicer, the RNase III enzyme that is evolutionarily conserved and contains helicase and PAZ domains, as well as two dsRNA-binding domains.
2001
Tuschl T and colleagues
First described RNAi in mammalian cells
2003
Paddison PJ, et al.
Sui G, et al.
Paul CP, et al.
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.
2003
Song E, et al.
First reported that siRNAs can be used therapeutically in whole animals.
Mammals
2004
Kawasaki H and Taira K
Morris KV et al.
First observed that siRNA silences gene at transcriptional level possibly through directing de novo DNA methylation.
2004
Acuity Pharmaceuticals
First phase I clinical trial of siRNA drug for age-related macular degeneration (AMD)
2006
Andrew Fire and Craig Mello
Won Noble Prize in Physiology or Medicine for discovering RNAi mechanism.
2006
Li LC, et al
First reported that small dsRNA induces transcriptional gene activation and coined the term "RNA activation" (RNAa).
2010
Davis ME et al.
First demonstrated that siRNA administered systemically to humans can produce a specific gene inhibition (reduction in mRNA and protein) by an RNAi mechanism of action.
Citation:
Wang, H. (2015, July 10). How RNA Works. Retrieved from http://www.umassmed.edu/rti/biology/how-rnai-works/
TAN, Fischer L and James Q YIN. "Rnai, A New Therapeutic Strategy Against Viral Infection". Cell Res 14.6 (2004): 460-466. Web. 18 May 2016.
TAN, Fischer L and James Q YIN. "Rnai, A New Therapeutic Strategy Against Viral Infection". Cell Res 14.6 (2004): 460-466. Web. 18 May 2016.
Timeline
Organism
Reference
1995
Guo S, and Kemphues KJ.
First noticed that sense RNA was as effective as antisense RNA for suppressing gene expression in worm
1998
Fire A, et al.
First described RNAi phenomenon in C. elegans by injecting dsRNA into C. elegans which led to an efficient sequence-specific silencing and coined the term "RNA Interference".
1999
Hamilton AJ and Baulcombe DC.
Reported posttranscriptional gene silencing (PTGS) in plants
Plants
2000
Zamore PD, et al.
Reported processing of long dsRNA by Rnase III (Dicer) into shorter fragments of 21-23-nt intervals in Drosophila extracts
2001
Bernstein E, et al.
Cloned Dicer, the RNase III enzyme that is evolutionarily conserved and contains helicase and PAZ domains, as well as two dsRNA-binding domains.
2001
Tuschl T and colleagues
First described RNAi in mammalian cells
2003
Paddison PJ, et al.
Sui G, et al.
Paul CP, et al.
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.
2003
Song E, et al.
First reported that siRNAs can be used therapeutically in whole animals.
Mammals
2004
Kawasaki H and Taira K
Morris KV et al.
First observed that siRNA silences gene at transcriptional level possibly through directing de novo DNA methylation.
2004
Acuity Pharmaceuticals
First phase I clinical trial of siRNA drug for age-related macular degeneration (AMD)
2006
Andrew Fire and Craig Mello
Won Noble Prize in Physiology or Medicine for discovering RNAi mechanism.
2006
Li LC, et al
First reported that small dsRNA induces transcriptional gene activation and coined the term "RNA activation" (RNAa).
2010
Davis ME et al.
First demonstrated that siRNA administered systemically to humans can produce a specific gene inhibition (reduction in mRNA and protein) by an RNAi mechanism of action.
Citation:
Wang, H. (2015, July 10). How RNA Works. Retrieved from http://www.umassmed.edu/rti/biology/how-rnai-works/
TAN, Fischer L and James Q YIN. "Rnai, A New Therapeutic Strategy Against Viral Infection". Cell Res 14.6 (2004): 460-466. Web. 18 May 2016.
TAN, Fischer L and James Q YIN. "Rnai, A New Therapeutic Strategy Against Viral Infection". Cell Res 14.6 (2004): 460-466. Web. 18 May 2016.